engleski

Production of paramylon, a beta-1, 3-glucan, by heterotrophic cultivation of Euglena gracilis on a synthetic medium

Euglena gracilis was cultivated on a synthetic medium of well-defined components. Biomass and paramylon (beta-1, 3-glucan) concentrations were the most important variables monitored. Mass production in the bioreactor was carried out following studies of operating conditions in shaken flasks. E. gracilis grew best at about 30 ^oC and at a low pH of 3. A pH control was not necessary, although the pH increased considerably at the end of the cultivation processes. Aeration could be performed at low stirrer frequency. Biomass concentrations of about 13– 14 g/L were obtained with paramylon mass fractions of 50– 60%, by starting with a synthetic medium containing 15 g/L of glucose as the main carbon source.

batch cultivation; Euglena gracilis; heterotrophic growth; synthetic medium

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