engleski

The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

The leaves of axenically grown Coleus blumei were inoculated with Agrobacterium rhizogenes strain A4 and hairy root clones were established. PCR and Southern hybridization analysis confirmed transgenic nature of hairy root clones. Cultures of normal roots, induced by  -naphthaleneacetic acid on leaf explants, and hairy roots were evaluated for growth and rosmarinic acid content. Significantly better growth and up to 2.8 higher amount of rosmarinic acid was detected by HPLC analysis in hairy root clones. The production of rosmarinic acid was changed by yeast extract or methyl jasmonate treatment. Methyl jasmonate stimulated rosmarinic acid accumulation in 6 out of 11 tested clones, while yeast extract induced RA accumulation in two and diminished it in 5 out of 11 tested hairy root clones.

Agrobacterium rhizogenes; elicitors; phenolic compounds; phenylalanine ammonia-lyase; transformation.

U radu je prikazan dio rezultata diplomskog rada Divora Kiseljaka.