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Activation of MAPK by potassium bisperoxo(1, 10-phenantroline)oxovanadate (V) (CROSBI ID 86481)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Cerovac, Željka ; Ban, Jasna ; Morinville, Anne ; Yaccato, Karin ; Shaver, Alan ; Maysinger, Dušica Activation of MAPK by potassium bisperoxo(1, 10-phenantroline)oxovanadate (V) // Neurochemistry international, 34 (1999), 4; 337-344-x

Podaci o odgovornosti

Cerovac, Željka ; Ban, Jasna ; Morinville, Anne ; Yaccato, Karin ; Shaver, Alan ; Maysinger, Dušica

engleski

Activation of MAPK by potassium bisperoxo(1, 10-phenantroline)oxovanadate (V)

Potassium bisperoxo(1, 10-phenantroline)oxovanadate (V) [bpV(phen)] is a potent proteine phosphatase inhibitor which mediates a variety of biological effects. The aim of these studies was to examine the role(s) of mitogen activated protein kinase (MAPK) pathways in PC12 cell proliferation and toxicity by bpV(phen). BpV(phen) exerts a biomodal effect in PC12 cells: proliferation at low and cell death at higher micromolar concentrations. Activation of MAPK by bpV(phen) depends on time and concentration. The phosphorylation pattern of extracelllular regulated kinases (ERK ˝), c-jun N-terminal activated kinases (JNK) and p-38 in PC12 cells is strikingly different. Activation of JNK is sustained in PC12 cells. In contrast, ERK 1/2 activation is transient and treatment with PD98059 indicates that ERK activation by bpV(phen) is partly independent from the ras-MEK pathway. Stability studies of bpV(phen) in DMEM and PBS showed linear relationship with T1/2 about 6 h and 10 days in DMEM and PBS, respectively. Comparison between the time courses of MAPK activation and kinetics of bpV(phen) decomposition as assessed by 51V-NMR analysis show that the initial and maximal phosphorylation signals are produced in the presence of the complex bpV(phen) and not caused by the decomposition products of bpV(phen).

PC12 cells; 51V-NMR; peroxovanadium complexes; JNK; ERK

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Podaci o izdanju

34 (4)

1999.

337-344-x

objavljeno

0197-0186

Povezanost rada

Biologija

Indeksiranost