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izvor podataka: crosbi

Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with F18ac+ nonenterotoxigenic E. coli vaccinal strain (CROSBI ID 152279)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Kovšca Janjatović, Ana ; Lacković, Gordana ; Božić, Frane ; Špoljarić, Daniel ; Popović, Maja ; Valpotić, Hrvoje ; Vijtiuk, Nada ; Pavičić, Željko ; Valpotić, Ivica Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with F18ac+ nonenterotoxigenic E. coli vaccinal strain // European journal of histochemistry, 53 (2009), 4; 189-198

Podaci o odgovornosti

Kovšca Janjatović, Ana ; Lacković, Gordana ; Božić, Frane ; Špoljarić, Daniel ; Popović, Maja ; Valpotić, Hrvoje ; Vijtiuk, Nada ; Pavičić, Željko ; Valpotić, Ivica

engleski

Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with F18ac+ nonenterotoxigenic E. coli vaccinal strain

Among animal diseases, colidiarrhea and colienterotoxemia caused by enterotoxigenic E. coli (ETEC) strains are the most prevalent porcine infections, commonly occurring in suckling and weaned pigs. The aim of this study was to test the immunogenicity and protective effectiveness of attenuated F18ac+ non-ETEC vaccine candidate strain against porcine colienterotoxemia by the quantitative phenotypic analysis of small intestinal leukocytes in weaned pigs. Additionally, we have evaluated adjuvanticity of levamisole as an immune response modifier. Following oral immunization with F18ac+ non-ETEC strain and challenge infection with F4ac+ ETEC strain, we have performed immunophenotipic localization and morphometric quantification of immune cell subsets in the small intestine of weaned pigs. The proportions of lymphoid CD3+, CD45RA+, CD45RC+, CD21+, IgA+ and myeloid SWC3+ cell subpopulations in the lamina propria and Peyer’ s patches of jejunum and ileum were evaluated by immunohistochemical avidin-biotin complex method and analyzed using software program Lucia G for digital image analysis. The numbers of these immune cells in the gut-associated lymphoid tissue were significantly increased in vaccinated/challenged pigs (p<0.05) compared to control nonvaccinated/challenge infected pigs. Numerically expressed data for jejunal/ileal cell subsets examined, demonstrated difference in capacity of protective immune responses between vaccinated and nonvaccinated, ETEC challenged pigs.

E. coli; gut immune cells; pigs; nonspecific/specific immunization;

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Podaci o izdanju

53 (4)

2009.

189-198

objavljeno

1121-760X

Povezanost rada

Veterinarska medicina

Indeksiranost