Progressive loss of lambda prophage recombinogenicity in UV-irradiated Escherichia coli : the role of RecBCD enzyme (CROSBI ID 86557)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Vlahović, Ksenija ; Petranović, Mirjana ; Zahradka, Davor ; Petranović, Drago
engleski
Progressive loss of lambda prophage recombinogenicity in UV-irradiated Escherichia coli : the role of RecBCD enzyme
RecBCD enzyme is involved in the radiation-induced process known as prophage inactivation. The process leads to the inability of lambda prophage to excise itself from the E. coli chromosome via site-specific recombination. In this work we sought to further characterize the role of RecBCD enzyme in this process. In addition, we examined the ability of irradiated prophage to recombine with the infecting homologous phage. We used several E. coli mutants differentially altered in RecBCD's activities. The results showed that in the mutants carrying either recB2109 or recD1903, which do not exhibit significant nuclease activities, the prophage progressively loses its capacity for both site-specific and general recombination. In the recB268 null mutant, however, prophage recombinogenicity remained fully preserved. We also showed that the prophage unable to recombine retained its ability to complement the mutant infecting phage and that the recombination frequencies in phage x phage crosses were not affected by postirradiation incubation. Our results suggest that the helicase activity of RecBCD is responsible for the progressive loss of prophage recombinogenicity. This loss is most probably a consequence of the unsuccessful RecBCD- dependent recombinational repair of double- stranded breaks in the cell chromosome, during which some structures unsuitable for further recombination reactions may be produced.
Escherichia coli ; lambda prophage ; DNA recombination ; recombinational DNA repair ; RecBCD enzyme
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Podaci o izdanju
151 (9)
2000.
727-738
objavljeno
0923-2508
1769-7123
10.1016/S0923-2508(00)01138-4