GDS(L) lipases from streptomycetes (CROSBI ID 551419)
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Podaci o odgovornosti
Marić, Marija ; Bielen, Ana ; Abramić, Marija ; Vujaklija, Dušica
engleski
GDS(L) lipases from streptomycetes
GDS(L) esterases/lipases investigated so far have very interesting biochemical and structural properties, but are still insufficiently studied. Furthermore, only a small number of streptomycete GDS(L) enzymes has been characterized so far. Three highly homologous GDS(L) lipases from genus Streptomyces were cloned: lipase from S. rimosus (Q93MW7, SrL) and two lipases from S. coelicolor (SCO1725, Sc1 and SCO7513, Sc2). Genes were PCR amplified and ligated into E. coli vector, resequenced, and subsequently ligated into the Streptomyces expression plasmid pANT849. Heterologous, lipase deficient host Streptomyces lividans was used enzyme production. We will present here the results of biochemical characterization of SrL and Sc1. Both enzymes are predominantly α -helical proteins, as shown by CD spectroscopy. They have high working temperature (55°C), stability over a broad range of pH and temperature, and can hydrolyze numerous classes of substrates (p-nitrophenyl alkanoates, α - and ß-naphthyl alkanoates, oils, triacylglycerols, and Tween detergents). The most pronounced difference between SrL and Sc1 is in acyl chain length specificity, as SrL shows maximal activity toward substrates with chain lengths C8-C10, and Sc1 shows preference for substrates with longer acyl-chain lengths (C14). Further, enzymes are stable and show activation in several tested organic solvents. These results implicate high biotechnological potential of the studied enzymes.
GDS(L) lipases; streptomycetes
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Podaci o prilogu
41-x.
2008.
objavljeno
Podaci o matičnoj publikaciji
Zbornik sažetaka znanstvenog simpozija 50 godina molekularne biologije u Hrvatskoj
Zahradka, Ksenija ; Plohl, Miroslav ; Ambriović-Ristov, Andreja
Zagreb: Institut Ruđer Bošković
978-953-6690-78-7
Podaci o skupu
Znanstveni simpozij 50 godina molekularne biologije u Hrvatskoj
poster
20.11.2008-21.11.2008
Zagreb, Hrvatska