engleski

ABC protein transport of MRI contrast agents in canalicular rat liver plasma vesicles and yeast vacuoles

The mechanisms of excretion into bile of hepatospecific Magnetic Resonance Imaging (MRI) contrast media employed labeled Gd-reagents EOB.DTPA, BOPTA, B 20790 (iopanoate-linked) and Gd-B 21690 (glycocholate-linked) for measurement in rat liver canalicular plasma membrane vesicles and yeast vacuoles. The presence of ATP gave threefold greater transport of B20790 and B21690 than of EOB.DTPA and BOPTA. In yeast vacuoles, the ATP stimulatory effect was eightfold with B 20790 and fivefold greater for B 21690, whereas in YCF1 or YLLO115w-deleted yeast cells the transport was significantly reduced and absent from duble mutants, YCF1 or YLLO115w. The transport was similar in wild-type and deletant cells for B 21690; taurocholate gave 85% inhibition. These data suggest that biliary secretion of structurally related MRI agents depend on molecular structure. The findings are suggestive as of possible value for clinical diagnosis of inherited hyperbilirubinemias and other disorders.

nije evidentirano

nije evidentirano