engleski

Primary lung cancer and TNF-alfa gene polymorphisms:A case-control study in a Croatian population

Tumor necrosis factor-a (TNF-a) is a multifunctional cytokine involved in the pathogenesis of various infl ammatory and malignant diseases. Previous studies investigating the role of the TNF-a gene polymorphisms in lung cancer have generated contradictory results. The present study investigated whether the TNF-a-308 and TNF-a-238 polymorphisms are associated with risk and/or severity of disease in Croatian lung cancer patients. This is the fi rst study in a Caucasian population to analyze the infl uence of these two polymorphisms on multiple types of lung cancer. Material/Methods: In a case-control study, lung cancer patients (n=230) and appropriate age- and sex-matched controls (n=230) were genotyped by the polymerase chain reaction/restriction fragment length polymorphism method. Allele and genotype frequencies were estimated by gene counting. The chisquared test was used to compare the observed numbers of different TNF-a genotypes for the population with those predicted by Hardy-Weinberg equilibrium. Differences in genotype and allele distributions in the patient and control groups were analyzed for statistical signifi cance using the chi-squared test or Fisher’ s exact test as appropriate. Results: There were no signifi cant differences in the genotype and allele frequencies for the TNF-a-308 and TNF-a-238 polymorphisms between lung cancer patients and controls. Furthermore, no association between the genotypes and different stages of lung cancer was detected. Conclusions: This study indicates that the TNF-a-308 and TNF-a-238 polymorphisms do not infl uence susceptibility to or severity of lung cancer in a Croatian population.

Lung cancer ; TNF-alfa polymorphism ; Tumor type ; Tumor stage ; Caucasian population ; Cancer susceptibility

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