engleski

Genetic characterization of mumps virus isolate during passaging in the amniotic cavity of embryonated chicken eggs

Due to the inherent genetic instability of RNA viruses, mumps virus contains a heterogeneous mixture of genomic RNA molecules with slightly different nucleotide sequences. Virus growth both in vitro and in vivo results in selection of different subpopulations, depending on growth conditions, In this work we describe the genetic characterization of mumps virus after adaptation of an alleged individual human isolate of mumps to the amniotic fluid of embryonated chicken eggs, Sequence analysis of small hydrophobic (SH) gene showed the presence of two dominant virus variants with nucleotide variation of 6% (variant A and variant B). Restriction fragment length polymorphism (RFLP) analysis showed a heterogeneous population of isolated viruses. Using specific primers for variant A and variant B, we did not confirm the presence of variant B in samples of variant A and vice versa. The results point out the investigated individual isolate contains two dominant virus variants and few minor substrains. It is very likely that the alleged individual isolate was contaminated early in the attenuation process (2nd or 3rd passage on eggs).

mumps virus; heterogeneous population; genetic characterization

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