engleski

Monitoring of early pregnancy and early embryonic mortality by ultrasound and determination of PAG and progesterone in cows

The aim of study was to investigate the role of pregnancy associated glycoproteins (PAG) in early pregnancy as well as possibility of using PAG and ultrasound as diagnostic tools in diagnosis of embrionic mortality. Our research included 74 simmental cows, 3-7 years old, which calved every year. According to ultrasound findings (17., 24., 35. and 45. day after AI) cows were divided in 3 groups: pregnant cows (n=34), nonpregnant cows (n=18) and cows suffered embryonic mortality (n=21). Blood samples were collected every 72 hours between 12. and 45. day after AI and level of progesterone and pregnancy associated glycoproteins (PAG) were determined. Statistical analysis of variance for progesterone at day 12., 21. and 35. after AI shown significant difference between pregnant cows and both nonpregnant and embryonic mortality group (P>0.05). PAG variance analysis at day 24., 30. and 34. after AI shown high significant difference (P>0.01) between the nonpregnant and both embryonic mortality and pregnant group. On the other hand, variance analysis has shown that middle values for PAG at days 40. and 45. after AI were high significantly different (P>0.01) between pregnant and both nonpregnant and embryonic mortality group. The conclusion came out that it is impossible to determine embryonic mortality just on basis of progesterone profile, but it is easy to distinguish pregnant from nonpregnant cows, if cows are supposed to be pregnant more than 21 day. It is very easy and accurate to distinguish nonpregnant cows from cows that suffered early embryonic mortality. Furthermore, 98% cows in our research who experienced embryonic mortality, lost embryo 17-24 days after AI, visible in drastic decrease of PAG 7.5-9.0 days later. Using PAG for pregnancy diagnosis enables us to prove existence of alive vital embryo in uterus 24 days after conception.

cow; PAG; progesterone; early pregnancv; embryo mortality

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