engleski

Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction

An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a high-specific immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the studied compouns, IAA and seven amino acid conjugates were analyzed in quantitie of fresh plant material as low as 30 mg. In seed of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmolg-1 fresh weight (IAA) to 0.44 pmolg-1 fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe, and Val from higher plants is reported here for the first time.

Immunoaffinity extraction; High-performance liquide chromatography (HPLC); Tandem mass spectrometry (MS/MS); Indole-3-acetic acid (IAA); Auxin conjugates; Helleborus niger L.

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