engleski

Occurrence of grapevine leafroll-associated virus-1 and 3 in Croatian autochthonous grapevine varieties from Dalmatia

Research for presence of Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) was conducted in 51 commercial vineyards in Dalmatian region included in mass positive clonal selection. Observed varieties were: Babica, Babic, Glavinusa, Grk, Ljutun, Marastina, Mladenka, Nincusa, Plavina, Plavac mali, Posip, Vlaska and Vugava. Detection of viruses was conducted using DAS-ELISA-test on cortical shavings taken from each individual plant included in investigation. Samples were collected during dormant period 2006 from main grape growing regions of each variety. The dominant virus was GLRaV-3 found in 885 of 1113 analyzed samples (79.52%) while incidence of GLRaV-1 was lower - found in 448 samples (40.25%). The infection rate with GLRaV-3 was: Babic 100%, Babica 100%, Glavinusa 100%, Grk 27.14%, Ljutun 100%, Marastina 79.55%, Mladenka 100%, Nincusa 100%, Plavac mali 68.31%, Plavina 25%, Posip 59, 22%, Vlaska 100% and Vugava 90.48%. The infection rate of GLRaV-1 was: Babic 4.08%, Babica 100%, Glavinusa 93.75%, Grk 0%, Ljutun 97.65%, Marastina 26.14%, Mladenka 35.00%, Nincusa 83.33%, Plavac mali 46.13%, Plavina 10%, Posip 39.81%, Vlaska 26.53% and Vugava 3.97%. In some varieties infection with both viruses was significant: Babica 100%, Ljutun 97.75%, Glavinusa 93.75%, while in other varieties was much more scarcer: Mladenka 35%, Plavac mali 30.28%, Vlaska 26.53%, Posip 24.27%, Nincusa 16.67%, Marastina 15.91%, Babic 4.08%, Vugava 3.18 %. In varieties Grk and Plavina same time infection with both viruses wasn't detected. Main symptoms of infection in form of downward rolling of leaves and earlier color changes was most evident on red berried cultivars in late summer and beginning of autumn, but latent infections were also frequent.

GLRaV-1; GLRaV-3; ELISA test; autochthonus grapevine varieties

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