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The effects of SMS 201-995 on anti CD3 or dexamethason-induced apoptosis in thymus (CROSBI ID 464057)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Trobonjača, Zlatko ; Radošević-Stašić, Biserka ; Rukavina, Daniel The effects of SMS 201-995 on anti CD3 or dexamethason-induced apoptosis in thymus // Immunology Letters (Abstract Issue) 56 (1997), 1-3 / Wagner, H. ; Heeg, K. ; Pfeffer, K. (ur.). Amsterdam: Elsevier, 1997. str. 245-x

Podaci o odgovornosti

Trobonjača, Zlatko ; Radošević-Stašić, Biserka ; Rukavina, Daniel

engleski

The effects of SMS 201-995 on anti CD3 or dexamethason-induced apoptosis in thymus

Introduction: Activation of immature thymocytes via TCR as well as exposure to glucocorticoids, such as dexamethasone, results in apoptosis. In vivo administration of anti-CD3 antibodies to mice induces programmed cell death predominantly in double positive (CD4+CD8+), CD3low cortical and CD3intermediate medullar cell subset, that is analogous to the antigen-induced negative selection of thymocytes, capable of recognizing self antigens. The main event in negative selection process is TCR-CD3 occupancy via TCR-MHC complex interactions, that leads to the clonal deletion. This process is additionally regulated through different intercellular contacts and humoral factors. Since the expression of the somatostatin gene mRNA was found in thymus epithelial cells that exhibit bell-shaped response to dexamethasone administration, in the present study we investigated the influence of somatostatin analogue SMS 201-995 on apoptosis induced by anti-CD3 antibodies or by dexamethasone in immature thymocytes of BALB/c mice. Material and methods: Phenotypic analysis of double positive cells and nucleic fragmentation in thymus of mice injected with 10 mg/kg/b.w. of SMS and anti-CD3 or dexamethasone were estimated 24, 48 and 72 hours, as well as, 5 and 15 days after treatment. Thymocyte phenotype was determined by direct immunofluerescence technique, and nucleic fragmentation by cell cycle analysis gating the region left from G0/G1 peak. Results: The data have shown that SMS can depress sensitivity to apoptosis of thymocytes induced by anti-CD3 antibodies. SMS reduced the decrease of double-positive cells induced with anti-CD3 injection. Additionally, the level of nucleic fragmentation was also reduced after combined treatment with SMS and anti-CD3. Conclusions: The data point to the possibility that somatostatin is involved in processes of thymocyte maturation and clonal deletion of self-reactive cells.

SMS 201-995; dexamethasone; thymus; anti CD3-induced apoptosis; mice

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Podaci o prilogu

245-x.

1997.

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objavljeno

Podaci o matičnoj publikaciji

Immunology Letters (Abstract Issue) 56 (1997), 1-3

Wagner, H. ; Heeg, K. ; Pfeffer, K.

Amsterdam: Elsevier

Podaci o skupu

13th European Immunology Meeting

poster

22.06.1997-25.06.1997

Amsterdam, Nizozemska

Povezanost rada

nije evidentirano