engleski

Genotoxicity of resin-based root canal sealers

Aim: To evaluate the in vitro genotoxicity and cytotoxicity of two different resin-based root canal sealers. Methodology: The sealers tested were: Epiphany (Pentron Clinical Technologies, Wallingford, CT, USA) and RealSeal (SybronEndo, USA). Each component of the material (Epiphany Primer, Epiphany Thinning Resin, Epiphany Sealant, RealSeal Primer, RealSeal Thinning Resin and RealSeal Root Canal Sealant), components in permutual combinations and all components mixed together were tested on human peripheral blood lymphocytes using the comet assay and ethidium bromide/acridine orange viability staining. Simultaneously, untreated negative control cultures were analysed in the same manner. DNA damage was evaluated following 4h treatment, and after 24h of repair period in the absence of any material’ s component. Results: After 4 hours treatment, except thinning resin, each tested component and different combinations of components induced significant increase in DNA migration ability (p<0.05). After 24 hours, combination of primer, thinning resin and sealant of both tested materials caused cell death inducing intense apoptosis. After the repair period, cells exposed to Epiphany Sealant and RealSeal Root Canal Sealant, both in polymerized and nonpolymerized form, exhibited control level of DNA damage. Conclusions: Under the conditions used in the study, primer and thinning resin of both resin- based root canal sealers and their combinations caused serious DNA damage mediated by their cytotoxicity, rather than genotoxicity.

genotoxicity; cytotoxicity

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