engleski

"Nozevit" - a new perspective for control of nosema disease

The Nosema disease is a parasitic disease of adult honey bees (Apis mellifera) caused by microsporidium Nosema sp. which in adverse living conditions forms spores. The disease occurs throughout the world, Croatia included, and it causes significant honey production and economic losses. Honey bees afflicted with nosemosis start to forge earlier, while pathological changes of their mid-gut epithelial cells, as well as digestive and metabolic disorders, cause malnutrition leading to premature deaths. The EU as well as Croatian regulations prohibit the use of antibiotics and Fumagilin in the treatment of apian diseases (EU3/01/081) because of potential development of resistance to used chemotherapeuticals, masking of the disease, possible relapses, as well as harmful residua of antibiotics and their secondary metabolites in the apian products. For that reason, the need arises for the production and utilization of natural phyto-pharmacological preparations in the treatment of the Nosema disease. The purpose of this work was to assess the effectiveness of the “ Nozevit” phyto-pharmacological preparation as a preventive measure during artificial invasion with Nosema sp. spores, as well as its effectiveness in the treatment of bee colonies affected with the Nosema disease. Nosema sp. spores for artificial infection were isolated from the positive collective sample of winter losses, submitted to laboratory examination in accordance with the provisions in force. The results of the first part of our study demonstrated a considerable reduction in spores number, respectively 48.73% on 10th day ; 50.46% on 15th day and 70.91% on 22nd day after artificial infection, in comparison with control group. The results of the second part of the study demonstrated that the treatment with “ Nozevit” first preventively and then curatively, had a reduced number of spores compared to the group which received the preparation only for curative purposes and compared to the number of spores counted at the beginning of the treatment (78.37% on 15th ; 75.47% on 20th and 23.92% on 25th day after the curative treatment). Nozevit exhibited a noticeable and significant effect against nosema infections under experimental conditions.

Nosema disease; antibiotics; herbal preparations; Nozevit

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