engleski

Spontaneous infection of Chelidonium majus and Ligustrum sp. with cucumber mosaic Cucumovirus (CMV) encapsidating satellite RNA

Background and purpose: Cucumber mosaic Cucumovirus (CMV) infects many plant species. Naturally occurring CMV is occasionally associated with a satellite RNA (satRNA) which is capable of modulating CMV-induced symptoms, making them more destructive to host plants. A lot of research has been done lately to investigate which plant species can be natural hosts or reservoirs for CMV-satRNA and if, how and when can those pathogens spread from their natural hosts to agronomically important species. Material and methods: CMV encapsidating satRNA was isolated from Chelidonium majus and Ligustrum sp. and studdied serologically, by means of double-stranded RNA (dsRNA) analysis and regarding reaction of mechanically inoculated test plants including agronomically important species. Results: The type of symptoms on mechanically inoculated experimental hosts implied CMV infection, which was confirmed by serological and dsRNA analysis. Agronomically important species like pepper, Swiss chard and spinach expressed severe mosaic after inoculation. Cucumber expressed systemic ring-shaped chlorosis. SatRNA was consistently present in dsRNA isolates from natural and experimental hosts except in Chenopodium quinoa in which satRNA replication was suppressed. Conclusions: Our results present the first report on Chelidonium majus and Ligustrum sp. as natural hosts of CMV encapsidating satRNA. Even symptomless Chelidonium plants were found to be natural hosts of those pathogens. Since virus with the associated satRNA caused significant symptoms on some vegetable species, potential spreading of those patogens from their natural, widely distributed hosts could cause serious epidemics.

Cucumber mosaic Cucumovirus (CMV); satellite RNA; Chelidonium majus; Ligustrum sp.

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