Stability of N-glycan profiles in human plasma (CROSBI ID 156129)
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Podaci o odgovornosti
Gornik, Olga ; Wagner, Jasenka ; Pučić, Maja ; Knežević, Ana ; Redžić, Irma ; Lauc, Gordan
engleski
Stability of N-glycan profiles in human plasma
Glycan heterogeneity was shown to be associated with numerous diseases and glycan analysis has a great diagnostic potential. Recently we reported high biological variability of human plasma N-glycome at the level of population. The observed variations were larger than changes reported to be associated with some diseases, thus it was of great importance to examine the temporal constancy of human N-glycome before glycosylation changes could be routinely analyzes in diagnostic laboratories. Plasma samples were taken from 12 healthy individuals. The blood was drawn on seven occasions during five days. N-linked glycans, released from plasma proteins, were separated using hydrophilic interaction high performance liquid chromatography into 16 groups (GP1-GP16) and quantified. The results showed very small variation in all glycan groups, indicating very good temporal stability of N-glycome in a single individual. Coefficients of variation from 1.6% for GP8 to 11.4% for GP1 were observed. The average coefficient of variation was 5.6%. These variations were comparable to those observed when analytical procedure was tested for its precision. Good stability of plasma N-glycome in healthy individuals implies that glycosylation is under significant genetic control. Changes observed in glycan profiles are consequence of environmental influences and physiologic responses and therefore have a significant diagnostic potential.
glycan analysis; human plasma; glycome stability; N-glycans/protein glycosylation
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Podaci o izdanju
Povezanost rada
Temeljne medicinske znanosti, Biologija