The link between survival and infectivity of Campylobacter jejuni cells under changing environmental conditions (CROSBI ID 555985)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Klančnik, Anja ; Vučković, Darinka ; Abram, Maja ; Smole Možina, Sonja
engleski
The link between survival and infectivity of Campylobacter jejuni cells under changing environmental conditions
Bacteria are exposed to different stress conditions in the environment, or during food production as well as in the host. Temperature changes, availability of nutrients or the presence of toxic substances and antimicrobials are some of them. Despite the lack of many stress response mechanisms that other food-borne bacteria possess, campylobacters display a distinct survival potential in the extraintestinal environment and consequently also high incidence as a cause of human infections. This is at least partially the consequence of microbial adaptation to environmental stresses (1, 2). Consequently, discovering the link between survival and infectivity is vital for risk assessment purposes and for establishing the dynamics of potential food-borne infection. Since there are not many publications in this field, we investigated C. jejuni following heat shock, starvation and oxidative stress. The environmental impact on physiology, survival and virulence properties were studied by culturability, viability, as well as the ability of adhesion, invasion and intracellular survival within in vitro cell culure model using Caco-2 intestinal cells and murine macrophages J774 (3). With additional in vivo experiments in the murine model we investigated the influence of Campylobacter stress response on its virulence potential. At different time points after infection bacterial spreading and tissue invasion of infected BALB/c mice was determined (2, 4). (1) Klancnik, A. et al.(2006):Int.J.Food Microbiol., 112:200-207. (2) Klancnik, A. et al.(2009):Res.Microbiol. (Article in press) (3) Sikic Pogacar, M. et al.(2009):Int.J.Food Microbiol., 129:68-73. (4) Vuckovic, D. et al.(1998):Microb.Pathog., 24:263-268.
Campylobacter; stress response; cell lines; mice
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Podaci o prilogu
182-x.
2009.
objavljeno
Podaci o matičnoj publikaciji
Acta Microbiologica et Immunologica Hungarica (Abstracts)
Marialigeti, K.
Budimpešta: Akadémiai Kiadó
Podaci o skupu
2nd Central European Forum for Microbiology (CEFORM)
predavanje
07.10.2009-09.10.2009
Keszthely, Mađarska