engleski

Mathematical model for Trametes versicolor growth in submerged cultivation

Trametes versicolor is a white-rot fungus known as a producer of extracellular enzymes such as laccase, mangane-peroxidase and lignin- peroxidase. The production of these enzymes requires detailed knowledge of the growth characteristics and physiology of the fungus. Submerged cultivations of T. versicolor on glucose, fructose and sucrose as sole carbon sources were performed in shake flasks. Sucrose hydrolysis catalyzed by the whole cells of T. versicolor was considered as one-step enzymatic reaction described with Michaelis-Menten kinetics. Kinetic parameters of invertase catalyzed sucrose hydrolysis were estimated (Km=7.99 g dm-3 and Vm=0.304 dm3 g-1 h-1). Monod model was used for description of kinetics of T. versicolor growth on glucose and fructose as sole carbon sources. Growth associated model parameters were estimated from the experimental results obtained by independent experiments ( =0.14 h-1, =8.06 g dm-3, =0.37 h-1 and =54.8 g dm-3). Developed mathematical model is in good agreement with the experimental results.

Trametes versicolor ; invertase kinetics ; biomass growth ; mathematical model

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