engleski

LOH of Rb1 gene in sporadic colon cancer

Colon cancer arises from the accumulation of mutations during progression from normal colon epithelium to adenoma and carcinoma. In addition to the activation of oncogenes, there is an inactivation of tumor suppressor genes via frequent allelic deletions. One of the tumour suppressor genes that are considered to be involved in the development of colon cancer is Rb1. The aim of this study was to evaluate the loss of heterozygosity at the polymorphic locus inside Rb1 gene in sporadic colon cancer. The investigated locus was a CTTT(T) repeat in intron 20. Normal tissue samples from 150 sporadic colon cancer patients were genotyped in order to determine the frequency of heterozygosity at this locus. Corresponding tumour tissue samples were then analyzed to determine the LOH status. We also compared the results between genders to find if the distributions were gender-related. The frequency of heterozygosity was 84% at the microsatellite locus in intron 20 and LOH was found in 9% of tumour samples. No significant differences were found between genders.

colon cancer; Rb1

nije evidentirano