engleski

Bone regeneration in mice deficient in functional B cells

Aim: to investigate bone regeneration after bone marrow ablation in mice deficient in functional B cells because of a microMT gene knockout. Method: Bone marrow of the wild-type and knockout mice was washed out with sterile saline after drilling a hole at the proximal end of the tibia. Bone histology and mRNA expression of bone related proteins were performed after 6, 9, and 12 days after bone marrow ablation. Results: In control wild-type mice, bone regeneration followed a typical course of endosteal bone proliferation followed by osteoclastic resorption and reconstitution of hemopoetic marrow. In micro MT mice the newly formed bone was more voluminous, there were more trabeculae that were denser and replaced the initial blood clot more quickly than in un wild-type mice. Whereas the mRNA for the bone specific protein (BSP) and collagen decreased from 6th to 12th day after marrow ablation in wild-type mice and knockout mice, mRNA for osteocalcin remained increased in the knockout mice in comparison to wild-type mice. Conclusion: B cells seem to be necessary for the control of new bone formation after bone marrow ablation, indicating that there is a fine balance between bone and bone mnarrow in mouse long bones.

B-lymphocytes; bone induction; cell differentiation; cytokines

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