engleski

Molecular characterization of phytoplasmas infecting grapevines in Croatia

Phytoplasmas, formerly called mycoplasma-like organisms (MLOs), from the class Mollicutes (prokaryotes simpler than bacteria), are known to be the causal agents of several hundred plant diseases worldwide, including yellows diseases of grapevine (Vitis vinifera L.). Visual symptom assessment, PCR amplification and RFLP (restriction fragment length polymorphism) analyses were used to detect and characterize phytoplasmas infecting grapevines in Croatia. Samples of various cultivars were collected from different viticultural areas in order to examine geographic distribution of phytoplasmas throughout the country. Only phytoplasmas belonging to Bois Noir subgroup (16SrXII-A or stolbur) were found in vineyards of continental Croatia. The fact that no phytoplasmas were detected in Dalmatia and Istria was in accordance with the absence of grapevine yellows symptoms in these regions.

phytoplasmas; grapevine yellows; geographic distribution; PCR; RFLP

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