engleski

Modelling of continuous of L-malic acid production by porcine heart fumarase and fumarase in yeast cells

Continuous production of L-malic acid will be presented in this paper. The fumarase isolated from porcine heart, fumarase in the permeabilized non- growing cells of baker's yeast and Saccharomyces bayanus (UVAFERM BC) were used as the biocatalysts. In the production of L -malic acid with fumarase isolated from porcine hearts, there was no enzyme deactivation for a period of two days. At the average residence time of 4 hours, the conversion of about 80 % was achieved. Deactivation of the enzyme was observed using permeabilized cells. This deactivation is described as reversible process. Conversion of about 50 % was achieved with the remaining enzyme activity. Mathematical model that describes the production of L-malic acid, which contains the enzyme deactivation rate, was developed. Based on simulations, used biocatalysts were compared. The results show that in the continuous production of L- malic acid, one milligram purified enzyme corresponds to 68 g (wet weight) cells of Saccharomyces bayanus or 120 g (wet weight) cells of baker's yeast.

L-malic acid ; fumarase ; baker's yeast ; permeabilization ; modeling

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