engleski

Studies of wilt resistance in hop

Hop production is an export oriented agricultural branch in Slovenia. Slovenia produces well known aromatic varieties, all bred at the Slovenian hop institute and well adapted to the specific microclimatic conditions. Because of the outbreak of wilt, the development of cultivars with resistance to Verticillium spp. is currently one of the main focuses in breeding. The use of marker-assisted selection (MAS) would greatly accelerate the development of wilt-resistant hop cultivars, so a research program has been set up to study gene(s) conferring resistance to V. albo-atrum and to develop resistance linked markers applicable in MAS. A genomic approach has been applied for mapping and cloning of gene(s) and we have so far developed various molecular markers, established family segregating for Verticillium resistance and constructed a genetic map. The research will continue with the development of markers linked to resistance gene(s) and isolation of genomic and cDNA clones, with validation of their (gene) function. In parallel, proteomics has been used to study the pathogenicity of V. albo-atrum and plant-pathogen interactions. Proteomic study of two V. albo-atrum pathotypes revealed differences which may in part explain the differences in their virulence and in an artificial infection experiment, two induced plant defence proteins were identified in hop xylem sap the function of which is being examined. We intend to continue proteomic work by focusing on identifying a higher number of induced xylem as well as root proteins and on their functional and validational analysis using a transformation system. We expect to identify proteins (genes) related to pathogenesis, which will allow us to interpret the hop-Verticillium interaction and further develop linked markers applicable for breeding hop cultivars tolerant to wilt.

fungi; plant diseases; Humulus lupulus

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