Biomimetic Organic-Inorganic Nanocomposite Coatings for Titanium Implants. In vitro and In vivo Biological Testing (CROSBI ID 159101)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Schade, R. ; Dutour Sikirić, Maja ; Lamolle, S. ; Ronold, H.J. ; Lyngstadass, S.P. ; Liefeith, K. ; Cuisinier, F. ; Füredi-Milhofer, Helga
engleski
Biomimetic Organic-Inorganic Nanocomposite Coatings for Titanium Implants. In vitro and In vivo Biological Testing
Recently described organic – inorganic nanocomposite coatings of the chemical composition: (PLL/PGA)10CaP[(PLL/PGA)5CaP]4 (coating A) and (PLL/PGA)10CaP[(PLL/PGA)5CaP]4(PLL/PGA)5, (coating B), applied to chemically etched titanium plates, have been tested by extensive cell culture tests and in vivo biological experiments, with uncoated titanium plates serving as controls. Before testing coated samples were stored for extended periods of time (from 2 weeks to 8 months) under dry, sterile conditions. Cells of the cell-lines MC3T3-E1 and/or SAOS-2 were used for the following cell culture tests: Initial adhesion (4 hours) and proliferation (up to 21 days), cell activity (XTT test), morphology, synthesis of collagen type I and alkaline phosphatase activity (all incubation up to 21 days). In addition coating B was tested against uncoated control in a validated in vivo pull-out model in rabbit tibia. The results of both in vitro and in vivo experiments show excellent biological properties of chemically etched titanium which are even surpassed by surfaces covered with coating B. Thus, after 8 weeks of healing the implants coated with B were significantly better attached to the cortical bone of rabbit thibiae than uncoated titanium controls with more than twice the force needed to detach coated implants. However, coating A (top crystal layer) had an adverse effect on both cell proliferation and activity, which is explained by morphological observations, showing inhibited spreading of the cells on its rough surfaces. The results also show the remarkable stability of the coatings when shelved under dry and sterile conditions.
organic – inorganic nanocomposite coatings
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
95A (3)
2010.
691-700
objavljeno
1549-3296
10.1002/jbm.a.32888