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Determining the population structure of Citrus tristeza virus - how many clones should be sequenced? (CROSBI ID 558932)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa

Černi, Silvija ; Ruščić, Jelena ; Šatović, Zlatko ; Krajačić, Mladen ; Škorić, Dijana Determining the population structure of Citrus tristeza virus - how many clones should be sequenced? // EMBO World Lecture Course VIRUS-HOST: Partners in Pathogenicity / Ramirez, Cecilia, Bonilla, José, Haenni, Anne-Lise, Lizano, Ana V. (ur.). 2010. str. 88-88

Podaci o odgovornosti

Černi, Silvija ; Ruščić, Jelena ; Šatović, Zlatko ; Krajačić, Mladen ; Škorić, Dijana

engleski

Determining the population structure of Citrus tristeza virus - how many clones should be sequenced?

According to quasispecies theory, the populations of RNA viruses display high genetic diversity. These dynamic populations are composed of a swarm of genomic variants that coexist within individual isolates. Genetic variants found within isolates of Citrus tristeza virus (CTV), the most devastating citrus pathogen, may be of different pathotype (have different pathogenic potential). Bearing in mind that minor population variants might influence symptom expression, and that the selection of variants during natural virus transmission is random, the information about virus population structure is of great epidemiological importance. The most reliable method used for the characterization of virus variants encompasses separation of individual variants by cloning, their identification and quantification by SSCP (single-strand conformation polymorphism) analysis and sequencing. However, in order to determine the virus population structure, authors sequence different number of clones, often only a few per isolate. The aim of this study was to evaluate whether sequencing greater number of clones results in better information about CTV pathotypes present in the population. Different pathotypes were detected by successive analysis of coat protein gene sequences of eight CTV isolates displaying different genetic diversity. Results suggest that, depending on a genetic diversity of the isolate, it is advisable to analyze up to 30 clones per sample, which is more than scientist usually do

CTV; quasispecies; pathotype

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Podaci o prilogu

88-88.

2010.

objavljeno

Podaci o matičnoj publikaciji

EMBO World Lecture Course VIRUS-HOST: Partners in Pathogenicity

Ramirez, Cecilia, Bonilla, José, Haenni, Anne-Lise, Lizano, Ana V.

Podaci o skupu

EMBO World Lecture Course VIRUS-HOST: Partners in Pathogenicity

poster

23.02.2010-26.02.2010

San José, Kostarika

Povezanost rada

Biologija