Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Nicotine content in transformed haploid and dihaploid tissues of tobacco (Nicotiana tabacum L.) (CROSBI ID 87529)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Ćurković Perica, Mirna ; Gillet, Francoise ; Jacquin-Dubreuil, Annie ; Krsnik-Rasol, Marijana ; Jelaska, Sibila Nicotine content in transformed haploid and dihaploid tissues of tobacco (Nicotiana tabacum L.) // Phyton (Horn), 37 (1998), 2; 229-239-x

Podaci o odgovornosti

Ćurković Perica, Mirna ; Gillet, Francoise ; Jacquin-Dubreuil, Annie ; Krsnik-Rasol, Marijana ; Jelaska, Sibila

engleski

Nicotine content in transformed haploid and dihaploid tissues of tobacco (Nicotiana tabacum L.)

Three anther-derived haploid and three haploid-derived dihaploid tissue lines of tobacco were transformed with three strains of Agrobacterium tumefaciens: B6S3, pGV2215 and pGV2255. Unorganized, shooty and rooty tumours were induced, respectively. Tumour morphology and the growth rate of transformed tissues depended mainly on the bacterial strain. The presence of T-DNA genes in transformed tissues was confirmed by polymerase chain reaction (PCR). By means of high performance liquid chromatography (HPLC) nicotine was found only in the control plants and in the roots transformed with Agrobacterium tumefaciens pGV2255. Its content did not depend on the ploidy level of the tissue. Transformed roots could not be subcultured because of callogenesis. The callus which overgrew transformed roots did not produce nicotine.

dihaploids; haploids; Nicotiana tabacum; nicotine content; transformation

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

37 (2)

1998.

229-239-x

objavljeno

0079-2047

Povezanost rada

Biologija

Indeksiranost