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Validation of an enzyme-linked immunosorbent assay for qualitative screening of neomycin in muscle, liver, kidney, eggs and milk (CROSBI ID 160436)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Solomun, Božica ; Bilandžić, Nina ; Varenina, Ivana ; Scortichini, Giampiero Validation of an enzyme-linked immunosorbent assay for qualitative screening of neomycin in muscle, liver, kidney, eggs and milk // Food additives and contaminants part A. Chemistry analysis control exposure & risk assessment, 28 (2011), 1; 11-18. doi: 10.1080/19440049.2010.527376

Podaci o odgovornosti

Solomun, Božica ; Bilandžić, Nina ; Varenina, Ivana ; Scortichini, Giampiero

engleski

Validation of an enzyme-linked immunosorbent assay for qualitative screening of neomycin in muscle, liver, kidney, eggs and milk

A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) was used for the qualitative screening analysis of neomycin in food of animal origin (muscle, liver, kidney, eggs and milk) at level corresponding to the European Union (EU) maximum residue limit (MRL) set for this substance. The method validation was performed according to the criteria of Commission Decision 2002/657/EC established for qualitative screening methods. In this regard, the following parameters were determined: detection capability (CCβ), specificity, detection limit (LOD), quantitation limit (LOQ), recovery, within-laboratory reproducibility, linearity and ruggedness. LOD values ranged from 5.7 μg kg−1 in kidney to 29.3 μg kg−1 in milk and LOQ values ranged from 11.4 μg kg−1 in kidney to 59.7 μg kg−1 in eggs. The recoveries from spiked samples at the MRL, half the MRL and double the MRL levels ranged from 59.2% to 122.8%, with a coefficient of variation (CV) between 4.6% and 28.6%. The CCβ value was less than the MRL for all examined matrices. Moderate variations of some critical factors in the sample pre-treatment for muscle, milk and eggs were deliberately introduced for ruggedness evaluation and had a slight effect on method performance. The proposed method is suitable for qualitative screening analysis of neomycin in the above-mentioned food in conformity with current EU performance requirements.

neomycin ; muscle ; kidney ; liver ; eggs ; milk ; enzyme-linked immunosorbent assay

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Podaci o izdanju

28 (1)

2011.

11-18

objavljeno

1944-0049

1944-0057

10.1080/19440049.2010.527376

Povezanost rada

Javno zdravstvo i zdravstvena zaštita

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