engleski

ESR1, LPL and APOE gene variants in relation to lipid status and obesity in young healthy subjects

Human obesity is a multifactorial syndrome influenced by both environmental and genetic factors. Among gene variants found to be involved in body weight regulation and development of obesity, particular attention has been paid to polymorphisms in genes related to adipogenesis, energy expenditure, and insulin resistance. We explored the association of genetic polymorphisms of: PPARG2, Pro12Ala ; adiponectin (ADIPOQ -11391G>A and 11377C>G) ; IL-6-174G>C ; estrogen receptor (ESR1alfa-TA): APOE ; ACE (I/D) ; MTHFR-677C>T ; LPL (PvuII+/-), with clinical variables: gender, age, BMI, and biological variables: triglycerides, cholesterol, HDL, LDL, CRP, homocysteine, glucose, in 105 healthy young subjects, (20-35 y) of Croatian origin. Genotyping of PPARG2, IL-6, ACE, LPL was performed by PCR-RFLP, APOE, MTHFR, ADIPOQ, by real-time PCR and ESR1alfa by capillary electrophoresis. Associations were performed of alleles, genotypes and haplotypes with biological variables. BMI was increased (>25) in 23% of subjects. Increased cholesterol values (>5.0 mmol/L) were found in 23% of subjects, LDL (>3.0 mmol/L) in 23%, triglycerides (>1.7 mmol/L) in 11.4% of subjects. We found statistically significant differences in subjects' weight (p=0.015), BMI (p=0.023), and hip/waist ratio (p=0.015) in regard to their diet type ; subjects with Mediterranean diet had the lowest values compared to continental and mixed diet. Significant associations were found for: LPL (PvuII+) genetic polymorphic variants and abdominal obesity (p=0.018) ; APOE4 variant and high LDL (p=0.0017) ; ESR1-L allele and hipercholesterolemia (p= 0.023). LPL, APO E and ESR1 genetic polymorphic variants represent predictive genetic risk markers for lipid status and obesity in young healthy subjects.

gene variants; obesity; young subjects

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