hrvatski

Lokusi kvantitativnih svojstava (QTL) za akumulaciju kadmija i cinka u listu kukuruza

Recent studies demonstrate that two metal pumps responsible for the loading of zinc ions (Zn2+) into xylem are also responsible for accumulation of toxic heavy metal cadmium (Cd) in the shoot. It is important, since mobility and availability of cadmium to crop plants pose risk to the food chain. Our objectives were to analyze variation for Cd and Zn concentrations in leaves of a maize mapping population and to detect the effects of quantitative trait loci (QTL) associated with the Cd and Zn concentrations. 290 F4 lines were phenotyped at two environments and genotyped with 121 polymorphic SNP and SSR markers evenly distributed across the genome. Cd and Zn accumulations in leaves of the population were significantly affected by the genotype. One major QTL affecting the Cd accumulation was detected on chromosome 2 associated with the SSR marker bnlg1831. The locus explained 49.8% of the phenotypic variation. The LOD score for the QTL of 32.5 indicates very high probability that this QTL strongly affected amounts of Cd in leaves. For zinc accumulation, one QTL was detected with the LOD score of just 3.30 with LOD threshold equivalent to an alpha = 0.10 genome-wide error rate. It explains 8.4% of the phenotypic variation. It suggests that zinc leaf accumulation is controlled by many more genes which were remained undetected in our study.

cink ; kadmij ; kukuruz ; list ; QTL

nije evidentirano

engleski

Quantitative trait loci (QTL) for accumulation of cadmium and zinc in maize leaves

Recent studies demonstrate that two metal pumps responsible for the loading of zinc ions (Zn2+) into xylem are also responsible for accumulation of toxic heavy metal cadmium (Cd) in the shoot. It is important, since mobility and availability of cadmium to crop plants pose risk to the food chain. Our objectives were to analyze variation for Cd and Zn concentrations in leaves of a maize mapping population and to detect the effects of quantitative trait loci (QTL) associated with the Cd and Zn concentrations. 290 F4 lines were phenotyped at two environments and genotyped with 121 polymorphic SNP and SSR markers evenly distributed across the genome. Cd and Zn accumulations in leaves of the population were significantly affected by the genotype. One major QTL affecting the Cd accumulation was detected on chromosome 2 associated with the SSR marker bnlg1831. The locus explained 49.8% of the phenotypic variation. The LOD score for the QTL of 32.5 indicates very high probability that this QTL strongly affected amounts of Cd in leaves. For zinc accumulation, one QTL was detected with the LOD score of just 3.30 with LOD threshold equivalent to an alpha = 0.10 genome-wide error rate. It explains 8.4% of the phenotypic variation. It suggests that zinc leaf accumulation is controlled by many more genes which were remained undetected in our study.

zinc ; cadmium ; maize ; leaf ; QTL

nije evidentirano