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Decreased IFN-γ production in infants with acute RSV infection related to lower TLR8 expression in NK cells (CROSBI ID 561153)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Bendelja, Krešo ; Vojvoda, Valerija ; Turkalj, Mirjana ; Tešović, Goran ; Rabatić, Sabina Decreased IFN-γ production in infants with acute RSV infection related to lower TLR8 expression in NK cells // 2010 Keystone Symposia Meeting Abstract Book. 2010. str. 74-x

Podaci o odgovornosti

Bendelja, Krešo ; Vojvoda, Valerija ; Turkalj, Mirjana ; Tešović, Goran ; Rabatić, Sabina

engleski

Decreased IFN-γ production in infants with acute RSV infection related to lower TLR8 expression in NK cells

Toll-like receptors (TLRs), as a part of the innate immunity, provide first line of defense able to activate immune system upon pathogen challenge. Respiratory syncytial virus (RSV) is single-stranded RNA virus which can cause severe lower respiratory tract infection (LRTI) in infants. TLR8 recognizes single-stranded viral RNA and initiates cascade of antiviral mechanisms necessary to limit virus spread and development of specific immune reaction. NK cells are among early responder cells in viral infections with ability to directly recognize and kill infected cells. Although NK cytolytic activity is mediated via killer activating receptors and FcγR’s it could be significantly up-regulated by IFN type I and II cytokines, in paracrine and autocrine fashion. Since peripheral blood NK cells express TLR8, we postulate that lower TLR8 receptor expression could be related to inability of NK cells to control RSV replication, to induce IFN-γ production and to participate in development of effective specific anti-RSV immune response in infants with RSV infection. In our study specific monoclonal antibody raised against human TLR8 was used to determine expression in peripheral blood NK cells from RSV-infected infants during acute infection, healthy infants and adults. Functional status of TLR8 in monocytes was assessed in vitro by intracellular IFN-γ upon TLR8-specific stimulation. Infants in acute phase of RSV infection had lower percentages of TLR8-expressing NK cells than healthy infants and adults. Lower TLR8 levels (mean fluorescence intensity) in NK cells during acute RSV-infection were accompanied by lower TLR8-mediaded IFN-γ production. Moreover, NK cell percentages and expression levels of TLR8 negatively correlated with severity of infection, assessed as hospitalization duration. In conclusion, lower expression of TLR8 in NK cells from RSV-infected infants could imply inadequate engagement of antiviral innate immune mechanisms responsible for RSV replication control and generation of effective immune response that might lead to exaggerated inflammation frequently observed in infants.

RSV; TLR8; NK cells; infants; IFN-gamma

dodijeljena stipendija za pohađanje sastanka

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

74-x.

2010.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

2010 Keystone Symposia Meeting Abstract Book

Podaci o skupu

Keystone Symposia on Molecular and Cellular Biology - Innate Immunity: Mechanisms Linking with Adaptive Immunity

poster

07.06.2010-12.06.2010

Dublin, Irska

Povezanost rada

Temeljne medicinske znanosti, Kliničke medicinske znanosti