engleski

Apotosis of leukemic cells- a case report

Transformation of leukemic cells is linked to delay of maturation, delay in apoptosis and altered responsiveness to growth factors. However, in some studies were found that FAS (CD95/APO1) that mediates apoptotic signal is more frequently expressed in M4/M5 leukemic cells, and could be related to poor clinical outcome of acute myeloid leukemia (AML) patients. Apoptosis of bone marrow (BM) leukemic cells in our patient with AML-M5 could be also related to FAS expression on leukemic cells. The aim of the study was to compare cytomorphology and cytochemistry of BM apoptotic leukemic cells to preserved peripheral blood (PB) leukemic cells in our patient with AML-M5. Patients and methods: In the study is reported a 76 years old men with AML-M5 treated at Zagreb University Hospital Center. BM and PB of AL patient were analyzed after Pappenheim and cytochemical stainings and classified according FAB and WHO classification. Flow cytometry and cytogenetic analyses of BM cells were also done. Results: In BM were found numerous apoptotic cells with characteristic nuclear chromatin condensation and formation of pyknotic nuclei and only few preserved monoblasts and promoncytes. Analysis of PB revealed leukocytosis and 80-90% monocytic cells (46% monoblasts, 29% promonocytes and 11% monocytes). In almost all of BM apoptotic leukemic cells and PB leukemic cells esterase was strongly positive. Conclusion: Cytomorphology and cytochemistry of PB leukemic cells point to proliferation of immature monocytic cells and cytomorphology of BM to cell apoptosis. Cytochemistry of BM apoptotic cells confirmed monocytic cell lineage and AML-M5b was diagnosed in our patient.

apototosis ; acute leukemia

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