engleski

Heterotrophic cultivation of algae Euglena gracilis and production of β-1, 3-glucan

The main goal of this research was to define the most favorable cultivation procedure for production of β-1, 3-glucan (paramylon) by heterotrophic cultivation of algae Euglena gracilis in stirred tank bioreactor. Paramylon is biologicaly active polysaccharide possessing imunostimulating and an antitumor effect, lowers the blood cholesterol level and his sulphur derivates inhibit the HIV-virus activity. In this investigation algae E. gracilis was cultivated on synthetic (Hutner) medium with different carbon sources (glucose, ethanol and Na-acetate) as well as on complex medium with different industrial by-products (e.g. molasses and corn steep liquor). Batch and continuous cultivation techniques were used for E. gracilis cultivation. The highest bioprocess efficiency parameters were observed during batch cultivation on complex medium with 20 g/L glucose and 35g/L corn steep liquor.

heterotrophic cultivation; algae Euglena gracilis; production of β-1; 3-glucan

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