engleski

Determination of multi-class pharmaceuticals in wastewater by liquid chromatography-tandem mass spectrometry (LC-MS-MS)

An analytical method for multi-class pharmaceuticals determination in wastewater has been developed and validated. Target compounds were: sulfonamides (sulfadiazine, sulfaguanidine, sulfamethazine, sulfamethoxazole), fluoroqinolones (ciprofloxacine, enrofloxacine, norfloxacine), diaminopyrimidine (trimethoprim), anesthetic (procaine), anthelmintic (praziquantel and febantel) and macrolide (roxithromycine). The method involves pre-concentration and clean-up by solid phase extraction (SPE) using Strata-X extraction cartridges at pH 4.0. Target analytes were identified and quantitatively determined by liquid chromatography-tandem mass spectrometry using multiple reaction monitoring (MRM). Recoveries were higher than 50% with relative standard deviation (RSD) below 18.3 % for three concentration levels. Only for sulfaguanidine low recovery was obtained. Matrix effect was evaluated using matrix-matched standards. The method detection limit (MDL) was between 0.5 and 5 ng/L in spiked water samples. The precision of the method, calculated as relative standard deviation, ranged from 0.5 to 2.0% and from 1.4 to 8.3 for intra- and inter-day analysis, respectively. The described analytical method was applied for determination of pharmaceuticals in effluent wastewaters from pharmaceutical industry.

pharmaceuticals ; wastewater ; liquid chromatography-tandem mass spectrometry ; solid phase extraction

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