engleski

Production of lignolytic enzymes by Phanerochaete chrysosporium

Nowadays, more attention is concerned to the environment, its preservation and sustainable development. Various industries are one of the greatest environmental pollutants, especially wood and paper industry. Many industries use dangerous chemicals for degradation of substances, such as paper industry in the process of lignin degradation. Due to these facts as well as world trends, these industries search alternative processes and example of this is lignin degradation by white rot fungi that produce lignolitic enzymes. In this work production of lignolitic enzymes by cultivation of the Phanerochaete chrysosporium fungi on different wastes was investigated. The wood industry waste, paper industry waste and methanogenic fraction of municipal waste were used as substrates. Phanerochaete chrysosporium is a white rot fungus, which in the secondary phase of its metabolism degrades lignin and aromatic pollutants that can be found in the materials contaminated with explosives, pesticides, and wastewater. It secretes all three lignolitic enzymes, lignin peroxidase, manganese peroxidase and laccase. Lignolitic extracellular enzymes are nonspecific and participate in various oxidation reactions that break down the aromatic structure of lignin as well as links between the basic subunits. The resulting lower molecular weight compounds can then be transported inside the cell and further degraded. The goal of the experiment was to define the influence of various supstrates on the cultivation of Phanerochaete chrysosporium in order to achieve maximum enzyme activity. The experiment was carried out in shake flasks. The enzyme volume activity is measured spectrophotometrically. Black grove, the waste from the paper industry, has shown the greatest potential for the production of lignolitic enzymes, but further research and optimization of process conditions, primarily of the reactive compound composition, are necessary to achieve significant activities.

manganese peroxidase; lignin peroxidase; laccase; white rot fungi; Phanerochaete chrysosporium

Ovaj se tekst temelji na radu koji je financirala Nacionalna zaklada za znanost, visoko školstvo i tehnologijski razvoj Republike Hrvatske.