engleski

Antigen doses used for challenge in mice model of asthma differently affect airway hyperresponsiveness and airway inflammation in the lungs

Airway inflammation (AI) and hyperresponsiveness (AHR) are two asthma hallmarks. However, the function of particular cell types, i.e. eosinophils or T-cells, in the development of AHR is still not unambigously elucidated. We hypothesized that challenge of mice with varying antigen doses could provide new insights into the complex mechanisms by which AI enhances AHR. AHR was detected only in mice challenged with larger OVA dose, indicating dependence of AHR on the antigen dose used for challenge. In contrast to AHR, accumulation of inflammatory cells was not influenced by applied antigen dose. Subsequent DNA microarray analysis revealed that larger OVA dose induces more prominent expression of macrophage chemoattractant protein 1 (MCP-1) and secreted phosphoprotein 1 (Spp1). Our results imply that AHR is more sensitive to effects of antigen dose than accumulation of inflammatory cells.

asthma; inflammation; hyperresponsiveness; ovalbumin; microarray; MCP-1; Spp1

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