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On Relevance of Codon Usage to Expression of Synthetic and Natural Genes in Escherichia coli (CROSBI ID 164894)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Supek, Fran ; Šmuc, Tomislav On Relevance of Codon Usage to Expression of Synthetic and Natural Genes in Escherichia coli // Genetics, 185 (2010), 1129-1134. doi: 10.1534/genetics.110.115477

Podaci o odgovornosti

Supek, Fran ; Šmuc, Tomislav

engleski

On Relevance of Codon Usage to Expression of Synthetic and Natural Genes in Escherichia coli

A recent investigation concluded that codon bias did not affect expression of green fluorescent protein (GFP) variants in Escherichia coli, while stability of an mRNA secondary structure near the 5' end played a dominant role. We demonstrate that combining the two variables using regression trees or support vector regression yields a biologically plausible model with better support in the GFP dataset and in other experimental data: codon usage is relevant for protein levels if the 5' mRNA structures are not strong. Natural E. coli genes had weaker 5' mRNA structures than the examined set of GFP variants, and did not exhibit a correlation between the folding free energy of 5' mRNA structures and protein expression.

codon bias; mRNA secondary structure; protein translation; heterologous expression; Escherichia coli

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Podaci o izdanju

185

2010.

1129-1134

objavljeno

0016-6731

10.1534/genetics.110.115477

Povezanost rada

Računarstvo, Biotehnologija, Biologija

Poveznice
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