Loss of heterozygosity of CDKN2A (p16INK4alpha) and RB1 tumor suppressor genes in testicular germ cell tumors (CROSBI ID 164963)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Vladušić, Tomislav ; Hrašćan, Reno ; Pećina-Šlaus, Nives ; Vrhovac, Ivana ; Gamulin, Marija ; Franekić, Jasna ; Krušlin, Božo
engleski
Loss of heterozygosity of CDKN2A (p16INK4alpha) and RB1 tumor suppressor genes in testicular germ cell tumors
Testicular germ cell tumors (TGCTs) are the most frequent malignances in young adult men. The two main histological forms, seminomas and nonseminomas, differ biologically and clinically. pRB protein and its immediate upstream regulator p16INK4a are involved in the RB pathway which is deregulated in most TGCTs. The objective of this study was to evaluate the occurrence of loss of heterozygosity (LOH) of the CDKN2A (p16INK4a) and RB1 tumor suppressor genes in TGCTs. Forty TGCTs (18 seminomas and 22 nonseminomas) were analyzed by polymerase chain reaction using the restriction fragment length polymorphism or the nucleotide repeat polymorphism method. LOH of the CDKN2A was found in two (6%) out of 34 (85%) informative cases of our total TGCT sample. The observed changes were assigned to two (11%) nonseminomas out of 18 (82%) informative samples. Furthermore, LOH of the RB1 was detected in two (6%) out of 34 (85%) informative cases of our total TGCT sample. Once again, the observed changes were assigned to two (10.5%) nonseminomas out of 19 (86%) informative samples. Both LOHs of the CDKN2A were found in nonseminomas with a yolk sac tumor component, and both LOHs of the RB1 were found in nonseminomas with an embryonal carcinoma component. The higher incidence of observed LOH in nonseminomas may provide a clue to their invasive behavior.
loss of heterozygosity ; CDKN2A ; RB1 ; seminomas ; nonseminomas
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nije evidentirano
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Podaci o izdanju
44 (3)
2010.
168-173
objavljeno
1318-2099
1581-3207
10.2478/v10019-010-0035-7
Povezanost rada
Biologija, Temeljne medicinske znanosti, Biotehnologija