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Some physicochemical characteristics of native and formaldehyde treated tobacco streak ilarvirus particles

The electrophoretic heterogeneity (three electrophoretic components) of native tobacco streak ilarvirus (TSV) preparations was demonstrated by gel immunoelectrophoresis. Formaldehyde (FA) fixation provoked enhancement of anode electrophoretic mobility and the gradual formation of homogeneous electrophoretic pattern. Following FA fixation the three main sedimenting zones (T, M, B) revealed a certain loss of virus material as compared with unfixed, freshly purified TSV; simultaneously the nucleoprotein level in additional underlying zone(s) enhanced showing of aggregate formation. A slight shift in virus sedimentation rate towards the bottom of the gradient was another effect of FA fixation. Preparations exposed to FA for a longer time revealed a sedimentation shift in the opposite direction. A drastic decline of the main sedimenting nucleoprotein components was demonstrated with unfixed TSV for several days following purification; the B zone showed the greatest tendency to virus loss. Electron microscopy and antigenicity analysis tests confirmed that the TSV aggregation occured in preparations treated by formaldehyde. The dynamics of virus aggregate formation was monitored by immunodiffusion tests.

tobacco streak ilarvirus; formaldehyde fixation; electrophoresis; sedimentation

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