Short-term salt and osmotic stress in Mamillaria gracillis Pfeiff. tissue culture (CROSBI ID 565009)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Balen, Biljana ; Rogić, Tea ; Šimac, Matija ; Peharec, Petra ; Bar-Zvi, Dudy ; Krsnik-Rasol, Marijana
engleski
Short-term salt and osmotic stress in Mamillaria gracillis Pfeiff. tissue culture
In vitro propagated M. gracilis plants develop calli without any exogenous growth regulators. This habituated calli spontaneously regenerate morphologically normal and hyperhydric shoots. Since the habituation and hyperhydricity are both part of a neoplastic progression, cactus cells were transformed with A. tumefaciens strain B6S3. Tumor line, which was established, never expressed any morphogenic capacity. The aim of this study was to investigate the effect of short-term salt and osmotic stress on the M. gracilis callus and tumor culture. Tissue explants were grown in a liquid nutrient and exposed for 15 min and 3 h to growth medium supplemented with 250 mM NaCl or 500 mM mannitol. Following treatments, tissue was collected and protein extracts were prepared. They were examined with regard to abundance and phosphorylation of ASR1 protein by western blotting and Pro Q Diamond fluorescent dye, respectively. Glycosylation pattern was examined by Pro Q Emerald fluorescent dye and lectin assay. ASR1 was present in both cactus tissues with three isoforms of approximately 42, 26 and 17 kDa. No difference in abundance or phosphorylation of ASR1 in callus and tumor was observed after either 15 min or 3 h treatment with salt. More intensive phosphorylation signal of ASR1 was noticed in callus grown on medium with mannitol for 3 h. Analysis of glycosylation pattern with lectins Con A and GNA indicated stronger protein glycosylation in callus exposed to mannitol after 15 min and 3 h.
salt stress; osmotic stress; cactus; plant tissue culture
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
P01-033.
2010.
objavljeno
Podaci o matičnoj publikaciji
FESPB 2010 Book of Abstracts
Beltran Maria, Jose Pio ; Rodriguez, Dolores
Valencia: FESPB
Podaci o skupu
FESPB 2010, XVII Congress of the Federation of European Societies of Plant Biology
poster
04.07.2010-09.07.2010
Valencia, Španjolska