engleski

Electrophoretic protein patterns of sugar beet tissue lines

Cellular and extracellular proteins accumulating in normal (hormone dependent), habituated and crown gall tumour callus lines of sugar beet (Beta vulgaris L. altissima) were studied. Proteins were separated by SDS-polyacrylamide gel electrophoresis and silver stained. Glycoproteins with D-manose in their glycan component were detected on protein blots by concanavalin A-peroxidase staining. A few tissue specific cellular proteins (16, 17, 18, 19, 24, 37 kDa) were detected. Patterns of proteins secreted in the nutrient medium of suspension cultured cells were distinct for each tissue line. Nonorganogenic habituated tissue contained more protein bands in the range between 25 and 66 kDa than the other lines. Among glycosilated extracellular proteins, the 68 kDa protein was common for all tissues, whereas the 45 kDa was distinct for both habituated lines. The nonorganogenic habituated tissue was deficient in the 34 kDa polypeptide, and the crown gall tumour in the 23 kDa one.

Beta vulgaris L.; Chewnopodiaceae; differentiation; extracellular glycoproteins; habituation; protein pattern; SDS-PAGE

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