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Haplotype analysis of 17 Y-short tandem repeat loci in western Croatia

Introduction: Y-chromosome haplotype reference database (YHRD) is very important in forensic community, because it stores Y chromosome haplotypes from global populations. The aim of this study was to perform the population genetic study on 17 short tandem repeat (STR) loci on the Y chromosome in western Croatia. Methods: We performed a statistical analysis of the data from previously performed genetic analysis collected during routine forensic work by the Forensic Science Centre “Ivan Vučetić”. A total of 220 unrelated men from western Croatia were selected for this study. Genomic DNA was extracted by Chelex from FTA cards. Y-chromosomal STRs were amplified using the AmpFlSTR Yfiler PCR amplification kit. Haplotype frequencies were analyzed using the Arlequin software v.3.1 and AMOVA analysis calculated with the YHRD online analysis tool. Results: Average locus diversity was 0.6038, and it ranged from 0.226 for DYS392 to 0.778 for DYS458. A total of 213 haplotypes were identified, 207 of which were unique. Total haplotype diversity was 0.994 and total discrimination capacity was 96.8%. AMOVA indicated that our western Croatian population was the closest to populations from Zagreb, Croatia and Mostar, Bosnia and Herzegovina. Conclusion: Future studies are needed to create a reference Croatian Y-STR database.

Y-chromosome; haplotype analysis; 17 Y-short tandem repeat loci; western Croatia

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