engleski

LOW RATE OF POINT MUTATION IN THE mtDNA NONCODING FRAGMENT OF HYPERICUM TAXA

Contrary to major impact on the study of phylogeny in animals and human, application of mitochondrial DNA (mtDNA) analyses in phylogenetic reseaches of plants is sporadic due to their high rate of rearrangmenets and low rate of point mutations in plants. Nevertheless, in some taxa mtDNA analysis gave interesting results. With aim to test variability and applicability of mtDNA in genus Hypericum, in this investigation 4 noncoding regions of mtDNA was compared using mtDNA RFLP on large number of Hypericum taxa. Out of thirthysix Hypericum taxa, bellonging to fifteen sections, and fifteen restiction profiles, difference in profiles of only three species were observed. One deletion was found in mtNAD1/Hinf I profile of H. pseudohenryi, while lose of restriction site was detected in mtNAD1/RsaI profile of H. olympicum and gain of restriction site in mtNAD1/RsaI profile of H. hirsutum. Such result confirms slow sequence evolution of the Hypericum mtDNA as is the case with the plant mtDNA in general.

mtDNA RFLP; Hypericum

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