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Determination of total phenols and flavonoids from sage (Salvia officinalis L.) using acid hydrolysis (CROSBI ID 566510)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Dent, Maja ; Dragović-Uzelac, Verica ; Penić, Marija ; Jukić, Marijana ; Halambek, Jasna ; Levaj, Branka Determination of total phenols and flavonoids from sage (Salvia officinalis L.) using acid hydrolysis // 15th World Congress of Food Science and Technology (IUFOST 2010 ) : Proceedings / Robertson, Gordon ; Lupien, John (ur.). IUFOST, 2010

Podaci o odgovornosti

Dent, Maja ; Dragović-Uzelac, Verica ; Penić, Marija ; Jukić, Marijana ; Halambek, Jasna ; Levaj, Branka

engleski

Determination of total phenols and flavonoids from sage (Salvia officinalis L.) using acid hydrolysis

Phenolic compounds were investigated in wild sage (Salvia officinalis L.). Conventional extraction (CE) in water bath shaking, extraction in an ultrasonic water bath (US) 35 kHz at 60 °C, 30 minutes and microwave assisted extraction (MAE) power 500 W at 60 °C, 10 minutes were compared. Extractions were performed using 30% ethanol and acetone with the use of acid hydrolysis with 10% and 20% HCl. Sage was harvested on the Mediterranean part of Croatia, dried in the shade and stored in paper bags. Total phenols (TP) were determined spectrophotometrically with Folin-Ciocalteu method. The flavonoids (FL) assay is based on the spectrophotometric evaluation of a complex with AlCl3. The influence of solvents on the amount of extracted TP and FL, and the application of acid hydrolysis with 10% and 20% HCl depending on the applied extraction were examined. Comparing the solvents used for extraction, 30% ethanol extracted higher amount of TP and FL than 30% acetone. Combined 30% ethanol as solvent with acid hydrolysis (10 % HCl) and MAE obtained the highest amount of TP (90.07 mg GAE / g dm), followed by US extraction (78.22 mg GAE/g dm), while CE produced lower amount of TP (62.78 mg GAE/g dm). Conclusions: Using MAE with acid hydrolysis the amount of TP and FL increased and was higher then the one obtained with CE. Application of acid hydrolysis with US extraction decreased the amounts of TP and FL. Extracts obtained with 30% ethanol and acetone without addition of HCl obtained approximately equal value of TP and FL. Acid hydrolysis with 10% HCl increased TP and FL, while the amounts decreased with application of 20% HCl .

sage; phenolic acid; flavonoids; acid hydrolysis

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Podaci o prilogu

2010.

objavljeno

Podaci o matičnoj publikaciji

15th World Congress of Food Science and Technology (IUFOST 2010 ) : Proceedings

Robertson, Gordon ; Lupien, John

IUFOST

978-0-9810247-0-7

Podaci o skupu

IUFoST 2010. 15 th World Congress of Food Science and Technology

poster

22.08.2010-26.08.2010

Cape Town, Južnoafrička Republika

Povezanost rada

Kemija, Prehrambena tehnologija