Determination of total phenols and flavonoids from sage (Salvia officinalis L.) using acid hydrolysis (CROSBI ID 566510)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Dent, Maja ; Dragović-Uzelac, Verica ; Penić, Marija ; Jukić, Marijana ; Halambek, Jasna ; Levaj, Branka
engleski
Determination of total phenols and flavonoids from sage (Salvia officinalis L.) using acid hydrolysis
Phenolic compounds were investigated in wild sage (Salvia officinalis L.). Conventional extraction (CE) in water bath shaking, extraction in an ultrasonic water bath (US) 35 kHz at 60 °C, 30 minutes and microwave assisted extraction (MAE) power 500 W at 60 °C, 10 minutes were compared. Extractions were performed using 30% ethanol and acetone with the use of acid hydrolysis with 10% and 20% HCl. Sage was harvested on the Mediterranean part of Croatia, dried in the shade and stored in paper bags. Total phenols (TP) were determined spectrophotometrically with Folin-Ciocalteu method. The flavonoids (FL) assay is based on the spectrophotometric evaluation of a complex with AlCl3. The influence of solvents on the amount of extracted TP and FL, and the application of acid hydrolysis with 10% and 20% HCl depending on the applied extraction were examined. Comparing the solvents used for extraction, 30% ethanol extracted higher amount of TP and FL than 30% acetone. Combined 30% ethanol as solvent with acid hydrolysis (10 % HCl) and MAE obtained the highest amount of TP (90.07 mg GAE / g dm), followed by US extraction (78.22 mg GAE/g dm), while CE produced lower amount of TP (62.78 mg GAE/g dm). Conclusions: Using MAE with acid hydrolysis the amount of TP and FL increased and was higher then the one obtained with CE. Application of acid hydrolysis with US extraction decreased the amounts of TP and FL. Extracts obtained with 30% ethanol and acetone without addition of HCl obtained approximately equal value of TP and FL. Acid hydrolysis with 10% HCl increased TP and FL, while the amounts decreased with application of 20% HCl .
sage; phenolic acid; flavonoids; acid hydrolysis
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Podaci o prilogu
2010.
objavljeno
Podaci o matičnoj publikaciji
15th World Congress of Food Science and Technology (IUFOST 2010 ) : Proceedings
Robertson, Gordon ; Lupien, John
IUFOST
978-0-9810247-0-7
Podaci o skupu
IUFoST 2010. 15 th World Congress of Food Science and Technology
poster
22.08.2010-26.08.2010
Cape Town, Južnoafrička Republika