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Analysis of the MCMV transcriptome reveals unexpected genome complexity (CROSBI ID 566698)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Vanda Juranić Lisnić, Marina Babić, Mišel Šatrak, Tihana Tršan, Stipan Jonjić, Joanne Trgovcich Analysis of the MCMV transcriptome reveals unexpected genome complexity // Godišnji sastanak Hrvatskoh imunološkog društva 2010. 2010

Podaci o odgovornosti

Vanda Juranić Lisnić, Marina Babić, Mišel Šatrak, Tihana Tršan, Stipan Jonjić, Joanne Trgovcich

engleski

Analysis of the MCMV transcriptome reveals unexpected genome complexity

Human cytomegalovirus (HCMV) is a widespread beta-herpes virus which, after mostly asymptomatic primary infection, establishes lifelong persistence with minimal or no damage to its host. However, reactivation of latent HCMV in immunosuppressed and immunologically immature individuals can lead to severe disease and even death, while the development of an effective HCMV vaccine is being hampered by our poor knowledge of the pathogenesis of HCMV infection and its species specificity. Nonetheless, murine cytomegalovirus (MCMV) is biologically similar and genetically strongly related to HCMV, making it an excellent model for in vivo studies of human-related CMV pathogenesis. Indeed, detailed characterization of various MCMV deletion mutants has led to the identification and characterization of new and significant immunoevasin genes. However, current genomic map of the MCMV was constructed by relying heavily on bioinformatic prediction of ORFs and their verification by genomic tilling arrays. Such an approach is problematic because it can lead to inaccurate annotation of ORFs, rare alternative splicing sites and transcript endpoints that could serve as targets for important regulatory molecules, for example miRNA. Therefore, the goal of this project was to characterize in detail all of the viral transcripts, both polyadenylated and non-polyadenylated, that accumulate in infected cells. Similar transcriptomic analysis of only poly(A) transcripts in HCMV revealed many novel genes and gene products, and our analysis revealed astonishing complexity of the MCMV transcriptome, which could not have been envisioned solely by in silico ORF prediction. We have detected a significant number of previously undetected transcripts and novel splice-variants, together with several S-AS pairs. Further characterization of these gene products may provide new insights into the mechanism of CMV diseases and represent novel targets in prevention and/or treatment of CMV infection.

MCMV; transcriptome; antisense transcription

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Podaci o prilogu

2010.

objavljeno

Podaci o matičnoj publikaciji

Godišnji sastanak Hrvatskoh imunološkog društva 2010

Podaci o skupu

Godišnji sastanak Hrvatskoh imunološkog društva 2010

predavanje

23.09.2010-26.09.2010

Mali Lošinj, Hrvatska

Povezanost rada

Temeljne medicinske znanosti