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Cultivation of bacterium Azotobacter chroococcum for biofertilizers preparation

Azotobacter chroococcum has ability to fix nitrogen from the atmosphere and therefore it could be used as an ingredient of biofertilizers. In order to use bacterial biomass for above mentioned purpose cultivation of A. chroococcum was done in different experimental conditions. During this research, optimization of chemically defined and complex media content were performed to define the most appropriate media content for bacterium cultivation in large scale. In this investigation, different cultivation techniques (batch, fed batch and repeated batch) were used for cultivation of bacterium A. chroococcum. The growth of bacterium A. chroococcum was monitored by determination of substrate and biomass concentration as well as other process parameters (e.g. pH, pO2 etc.). Comparison between different cultivation techniques was done on the basis of bioprocess efficiency parameters and it was observed that repeated batch technique is the most efficient and appropriate for use in industrial scale.

Azotobacter chroococcum; chemically defined and complex media; different cultivation techniques; biofertilizers

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