Linoleic acid peroxidation vs. isomerization : a biomimetic model of free radical reactivity in the presence of thiols (CROSBI ID 166888)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Mihaljević, Branka ; Tartaro, Ivana ; Ferreri, Carla ; Chatgilialoglu, Chryssostomos
engleski
Linoleic acid peroxidation vs. isomerization : a biomimetic model of free radical reactivity in the presence of thiols
Biomimetic models of free radical-induced transformation of PUFA, such as micelles and liposomes, have been used for the study of lipid peroxidation and lipid isomerization. Free radical reactivity of thiol compounds is the common link of the two processes, since lipid peroxidation is inhibited by thiols, due to their H-donating ability, whereas lipid isomerization is catalyzed by S-centered radicals. In this paper the two processes a compared for the first time, in solution and under biomimetic conditions, demonstrating that hydroperoxides and trans lipids are formed at comparable extent as result of oxidative free radical conditions. The biomimetic model of micelles of linoleic acid, prepared by addition of a non-ionic surfactant (TWEEN®-20) and 2-mercaptoethanol as the amphiphilic thiol, was irradiated by ionizing radiations up to 400 Gy under various conditions. In air-equilibrated solutions, the cis-trans isomerization process was observed with a catalytic cycle of 370 together with a substantial amount of hydroperoxides (LOOH). The effect of micelle size was also studied in order to envisage the effect of the supramolecular organization for the outcome of the two processes, and in particular, for the positional preference of the double bond isomerization.
lipid peroxidation; isomerization; linoleic acid; micelle
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