engleski

Iron-Mediated Lipid Peroxidation: Impact of molecular environment on the lipid oxidation induced by ferrous ion

The general objective of our part of the project would be to improve the understanding and detection of lipid peroxidation processes in simple model systems. The planned investigations of the kinetics and mechanisms of short-lived reactive species shall be extended to the study of biologically important molecules and processes whereby chemically reactive free radicals could be produced under controlled conditions. Since iron is one of the biological dominant transition metals, understanding of iron chemistry in different media is very important. The most common cause of lipid oxidation is the interaction between transition metals originating in the aqueous phase and lipid hydroperoxides located at the interface in lipid model systems (micelles). Information obtained in these systems may be applicable to the elucidation of the processes occurring in more complex biological media. The rate of various chemical reactions may be changed in micellar media due to the two most important factors, hydrophobic and/or electrostatic interactions of the surface of aggregates with reactive species in the aqueous phase. Further studies on the influence of interface characteristics on the oxidizability of polyunsaturated fatty acids in aqueous model systems could lead to better understanding of their oxidative stability as compared with the bulk phase or in solution. By measuring the kinetics of reactive species and concentrations of stable products formed we hope to contribute to: (a) the understanding of mechanisms of reactions of transition metals in different model media, with special attention given to reactions of iron with biologically important molecules and the formation and the reactions of free radicals ; (b) the improvement of the existing methods for the detection and quantitation of lipid peroxidation products.

Lipid peroxidation; Free radicals; Iron; Kinetic

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