engleski

4-Hydroxynonenal modulated cytokine production of in vitro blood-brain barrier

Brain tissue is particular susceptible to oxidative damage via oxygen radicals. The formation of free radicals and other oxidants is combined with an enhanced lipid peroxidation in cells and tissues. Among the end products of lipid peroxidation of particular relevance is 4- hydroxynonenal (HNE), which is present in the blood-brain barrier (BBB) under pathological circumstances, such as relative hypoxia or inflammation. Besides, HNE causes impairment of in vitro BBB model. However, HNE acts not only as a second messenger of free radicals, but also as a growth regulating factor interfering with the effects of various cytokines. Cells constituting BBB actively produce cytokines, therefore participate in mediation of immune response within central nervous system. In response to injury or infections, cytokines are released at high concentrations thus contributing to both neurodegeneration and to astrocytic reactivity. Hence, in vitro model of BBB was established to study effects of HNE on cytokine production of both endothelial and astrocytic side of BBB model by using Luminex x-map technology. HNE caused differences in production of CINC-2αβ, IL-1β and VEGF suggesting its role in modulation of inflammatory processes. Although expected, no differences in TNF α, IL-6 and IFN γ production occurred.

blood-brain barrier; HNE; lipid peroxidation; cytokine

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