engleski

Modulation of splenic and liver cell interactions in vitro by lipid peroxidation product 4-hydroxynonenal (HNE)

There are data indicating specific involvement of cytotoxic splenic cells in regulation of liver regeneration. On the other hand, otherwise harmful, oxidative stress was also shown to play a regulatory role in liver regeneration. The aim of our study was to analyse the response of liver cells on different concentrations of the cell growth modifying aldehyde HNE, known also as a „second toxic messenger of free radicals“, in combination with different concentrations of foetal calf serum (FCS). The major research scope was to investigate interactions between splenic and liver cells (primary cultures established from rat organs) in condition of oxidative stress simulated by HNE. Results showed that liver cell growth depends on concentration of FCS, and that high concentration of HNE inhibits the growth of the liver cells. The competition for 3H-thymidine incorporation in mixed cultures of liver and splenic cells revealed inhibiting effects of splenic cells on liver cells growth. However, if liver cells were pre-treated with HNE in presence of splenic cells the intensity of 3H-thymidine incorporation increased. From these results we can conclude that HNE is cytotoxic as well as cell growth modulating factor for liver cells and that splenic cells also inhibit liver cell growth, but not if the liver cells were pre-treated by HNE. There are two possibilities: either liver cells treated with HNE stimulated splenic cells to multiply (mitogen activation), or splenic cells helped recovery of liver cells damaged by HNE. Which one is correct should reveald our further studies.

oxidative stress; liver regeneration; splenic and liver cells interactions

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